Journal: BMC Evolutionary Biology

Article Title: Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system

doi: 10.1186/s12862-017-0904-4

Figure Lengend Snippet: Quantitative real-time PCR (qPCR) expression levels (shown as Fold Change, RQ) between biological groups (putative venom glands (pvg), pharyngeal lobes, and posterior body wall) of five analyzed GLTx transcripts (GLTx paralog 1–3, and adjacent gene regions GLTx-3’ and GLTx-5’; for details see Material and methods section “Quantitative real-time PCR”) in G. tridactyla ( n = 10; *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05). a Relative GLTx expression (logarithmic scale) in putative venom glands (grey) and pharyngeal lobes (orange) in comparison to the GLTx expression signal exhibited by the body tissue (RQ = 1). Relative GLTx expression in the pharyngeal lobes and putative venom glands is significantly different from the expression signal in the body tissue. b Relative GLTx expression (linear scale) within the pharyngeal lobes in comparison to the putative venom glands (RQ = 1). Relative GLTx expression is significantly different between both putative venom glands and pharyngeal lobes

Article Snippet: Quantitative real-time PCR (qPCR) experiments were performed on Glycera tridactyla Schmarda, 1861 (Annelida, Glyceridae) specimens obtained from the Roscoff marine biological station in February 2015.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Comparison

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the invasive ability of HCT-116 cells. (A) Microscopic observation and photography after crystalline violet staining (100× magnification); (B) the OD values of each group at 570 nm wavelength after dissolution of crystalline violet by sodium acetate; (C-G) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction; OD, optical density.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Staining, Dissolution, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the apoptosis of HCT-116 cells. (A,B) Flow cytometric detection of the effects of different transfection groups on apoptosis of HCT-116 cells; (C-I) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and Western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Frontiers in Physiology

Article Title: Ligand-binding properties of XaffOBP9, a Minus-C odorant-binding protein from Xyleborus affinis (Coleoptera: Curculionidae: Scolytinae)

doi: 10.3389/fphys.2023.1326099

Figure Lengend Snippet: The expression profiles of XaffOBPs using RNA-seq and RT-qPCR. Different lowercase letters indicate the significant differences in the expression level of XaffOBP s genes measured by RT-qPCR.

Article Snippet: The Real-time quantitative PCR (RT-qPCR) primers for the XaffOBPs genes were designed using Primer Premier v5.0 (Premier Biosoft, CA, United States) and are provided in . β-actin was employed as an internal reference.

Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR